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For the C26 model, eight-week-old BALB/c female and male mice were injected bilaterally at day 0 with a total 1×10 6 C26-cell allografts. Tibialis anterior (TA) muscle was collected in different cohorts after 10-, 20-, or 25-days following tumor inoculation or 25-days following PBS injections as a sham healthy control (n=6–8/group/sex). For the APC model, TA muscle was collected from 20-week-old Apc Min /+ female and male mice and their wild type-littermates (WT, C57bl/6 mice) as control (n=6–8/group/sex). RNA was extracted and sequenced to identify changes in the transcriptome. DNA was extracted and Reduced representation bisulfite <t>sequencing</t> <t>(RRBS)</t> was utilized to identified differentially methylated (DM) CpG sites. Data was integrated using BETA to assess the regulatory potential of DM regions on gene expression. Figure created with BioRender.com .
Representation Bisulfite Sequencing Rrbs, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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For the C26 model, eight-week-old BALB/c female and male mice were injected bilaterally at day 0 with a total 1×10 6 C26-cell allografts. Tibialis anterior (TA) muscle was collected in different cohorts after 10-, 20-, or 25-days following tumor inoculation or 25-days following PBS injections as a sham healthy control (n=6–8/group/sex). For the APC model, TA muscle was collected from 20-week-old Apc Min /+ female and male mice and their wild type-littermates (WT, C57bl/6 mice) as control (n=6–8/group/sex). RNA was extracted and sequenced to identify changes in the transcriptome. DNA was extracted and Reduced representation bisulfite <t>sequencing</t> <t>(RRBS)</t> was utilized to identified differentially methylated (DM) CpG sites. Data was integrated using BETA to assess the regulatory potential of DM regions on gene expression. Figure created with BioRender.com .
Representation Bisulfite Sequencing, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/representation bisulfite sequencing/product/Zymo Research
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For the C26 model, eight-week-old BALB/c female and male mice were injected bilaterally at day 0 with a total 1×10 6 C26-cell allografts. Tibialis anterior (TA) muscle was collected in different cohorts after 10-, 20-, or 25-days following tumor inoculation or 25-days following PBS injections as a sham healthy control (n=6–8/group/sex). For the APC model, TA muscle was collected from 20-week-old Apc Min /+ female and male mice and their wild type-littermates (WT, C57bl/6 mice) as control (n=6–8/group/sex). RNA was extracted and sequenced to identify changes in the transcriptome. DNA was extracted and Reduced representation bisulfite <t>sequencing</t> <t>(RRBS)</t> was utilized to identified differentially methylated (DM) CpG sites. Data was integrated using BETA to assess the regulatory potential of DM regions on gene expression. Figure created with BioRender.com .
Representation Bisulfite Sequencing Reduced Representation Bisulfite Sequencing Rrbs Library Preparation, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Zymo Research conventional bisulfite sequencing
For the C26 model, eight-week-old BALB/c female and male mice were injected bilaterally at day 0 with a total 1×10 6 C26-cell allografts. Tibialis anterior (TA) muscle was collected in different cohorts after 10-, 20-, or 25-days following tumor inoculation or 25-days following PBS injections as a sham healthy control (n=6–8/group/sex). For the APC model, TA muscle was collected from 20-week-old Apc Min /+ female and male mice and their wild type-littermates (WT, C57bl/6 mice) as control (n=6–8/group/sex). RNA was extracted and sequenced to identify changes in the transcriptome. DNA was extracted and Reduced representation bisulfite <t>sequencing</t> <t>(RRBS)</t> was utilized to identified differentially methylated (DM) CpG sites. Data was integrated using BETA to assess the regulatory potential of DM regions on gene expression. Figure created with BioRender.com .
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For the C26 model, eight-week-old BALB/c female and male mice were injected bilaterally at day 0 with a total 1×10 6 C26-cell allografts. Tibialis anterior (TA) muscle was collected in different cohorts after 10-, 20-, or 25-days following tumor inoculation or 25-days following PBS injections as a sham healthy control (n=6–8/group/sex). For the APC model, TA muscle was collected from 20-week-old Apc Min /+ female and male mice and their wild type-littermates (WT, C57bl/6 mice) as control (n=6–8/group/sex). RNA was extracted and sequenced to identify changes in the transcriptome. DNA was extracted and Reduced representation bisulfite sequencing (RRBS) was utilized to identified differentially methylated (DM) CpG sites. Data was integrated using BETA to assess the regulatory potential of DM regions on gene expression. Figure created with BioRender.com .

Journal: American journal of physiology. Cell physiology

Article Title: Skeletal Muscle Methylome-Transcriptome Disruptions During the Onset and Progression of Colorectal Cancer-Induced Cachexia

doi: 10.1152/ajpcell.00751.2025

Figure Lengend Snippet: For the C26 model, eight-week-old BALB/c female and male mice were injected bilaterally at day 0 with a total 1×10 6 C26-cell allografts. Tibialis anterior (TA) muscle was collected in different cohorts after 10-, 20-, or 25-days following tumor inoculation or 25-days following PBS injections as a sham healthy control (n=6–8/group/sex). For the APC model, TA muscle was collected from 20-week-old Apc Min /+ female and male mice and their wild type-littermates (WT, C57bl/6 mice) as control (n=6–8/group/sex). RNA was extracted and sequenced to identify changes in the transcriptome. DNA was extracted and Reduced representation bisulfite sequencing (RRBS) was utilized to identified differentially methylated (DM) CpG sites. Data was integrated using BETA to assess the regulatory potential of DM regions on gene expression. Figure created with BioRender.com .

Article Snippet: Reduced Representation Bisulfite Sequencing (RRBS) was performed by Zymo Research Corporation (Irvine, CA, USA) on 6–10 ng of DNA.

Techniques: Injection, Control, Methylation Sequencing, Methylation, Gene Expression